HPLC Columns and Cartridges
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MilliporeSigma™ Supelco™ Ascentis™ Express Biphenyl Column, 2.7 μm Particle Size
The Ascentis™ Express biphenyl phase chemistry offers alternative selectivity for pharmaceutical analytes and drug metabolites that are not well retained or difficult to resolve on traditional alkyl (C18) and phenyl bonded phases.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2.7 μm |
| Stationary Phase | Biphenyl |
| Phase | Reversed Phase |
| Matrix | Biphenyl Phase/Particle Platform (Fused-Core) |
| Endcapped | Yes |
| Material | Stainless Steel Hardware |
| Technique | Fused-Core HPLC |
| Product Line | Ascentis |
Thermo Scientific™ Acclaim™ VANQUISH™ Polar Advantage II UHPLC Columns
Achieve even greater power of separation, speed, and throughput of polar and nonpolar compounds in a single run.
| Pore Size | 120 Å |
|---|---|
| Stationary Phase | Polar Embedded |
| Temperature | 60°C |
| Phase | Reversed Phase |
| For Use With | LC/MS and RSLC |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| Surface Area | 300 m²/g |
| Max. Pressure | 21756 psi (1500 bar) |
| Column Format | Analytical Column |
| Particle Size | 2.2 μm |
| Carbon Load | 17% |
| Packing Material | Spherical, Fully Porous, Ultrapure Silica |
| Diameter (Metric) | 2.1 mm |
| pH | 1.5 to 10 |
| Technique | Bio-LC |
| Product Line | Acclaim |
| USP Type | L60 |
MilliporeSigma™ Supelco™ Ascentis™ C18 HPLC Column, 5 μm Particle Size
Ascentis™ C18 is an extremely stable and reliable HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.
| Pore Size | 100 Å |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | C18 (octadecyl) |
| Phase | Reversed Phase |
| Matrix | C18 (octadecyl) Phase/Fused-Core Particle Platform |
| Endcapped | Yes |
| pH | 2 to 8 |
| Technique | Fused-Core HPLC |
| Surface Area | 450 m2/g |
| Product Line | Ascentis |
TSKgel™ Phenyl-5PW RP HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel Phenyl-5PW RP offers similiar selectivity to its hydrophobic interation counterpart TSKgel Phenyl-5PW, although a higher degree of cross-linking allows for increased stability in aqueous/organic mobile phase gradients. Provides an advantage over silica when high pH buffer systems are needed.
| Shipping Condition | Shipped in 30% Methanol, 70% Water |
|---|---|
| Pore Size | 1000 Å |
| Particle Size | 10 μm |
| Solvents | 0-100% Organic Solvent |
| Ligand Type | 1 meq/mL (Estimated Ligand Density) |
| Temperature | 4°C to 60°C |
| Phase | Reversed Phase |
| Length (Metric) | 7.5 cm |
| pH | 2 to 12 |
| Product Line | TSKgel |
Thermo Scientific™ POROS™ CaptureSelect™ LC Lambda Affinty HPLC Columns
Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.
| Shipping Condition | Wet Ice |
|---|---|
| Pore Size | 2000 Å |
| Content And Storage | Column includes EZ grip fittings and column test certificate Store at 4°C. DO NOT FREEZE. |
| Target Molecule | Lambda Light Chain |
| Column Type | Affinity |
| Sample Type | Cell Culture Supernatants |
| Quantity | 1 Column |
| Material | PEEK |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
MilliporeSigma™ Supelco™ Ascentis™ Express HILIC HPLC Column, 5 μm Particle Size
The bare silica stationary phase of Ascentis™ Express 5 μm HILIC provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversed-phase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | Silica |
| Phase | Reversed Phase |
| Matrix | Silica Phase/Fused-Core Particle Platform |
| Endcapped | No |
| pH | 2 to 9 |
| Technique | Fused-Core HPLC |
| Product Line | Ascentis |
MilliporeSigma Ascentis™ Express PCS-Phenyl-Hexyl HPLC Column
Ascentis™ Express PCS-Phenyl-Hexyl columns are specifically engineered to provide efficient separation of basic, acidic, or neutral compounds under low ionic strength (formic acid) mobile phase conditions.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2.7 μm |
| Type | HPLC Column |
| Phase | Positively Charged Surface (PCS) |
| Material | Stainless Steel |
| Technique | HPLC |
MilliporeSigma™ Supelco™ ChromegaChiral™ Chiral CCO HPLC Column, 10 μm Particle Size
ChromegaChiral™ CCO is a polysaccharide coated chiral stationary phase and columns which are produced using a unique production process of coating the proven chiral selector, tris-(3,5-di-methylphenyl) carbamoyl cellulose on high purity, high performance silica.
| Pore Size | 1000 Å |
|---|---|
| Particle Size | 10 μm |
| Stationary Phase | 2-fluoro 5-methylphenyl cellulose |
| Phase | Chiral |
| Matrix | 2-fluoro 5-methylphenyl cellulose Phase |
| Technique | Chiral HPLC |
| Product Line | ChromegaChiral |
TSKgel™ SuperHZ1000 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel SuperHZ1000 Columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of solvent-soluble small molecules and oligomers less than 1,000 Da.
| Pore Size | 15 Å |
|---|---|
| Particle Size | 3 μm |
| Stationary Phase | Polystyrene-divinylbenzene |
| Temperature | 60°C |
| Matrix | Polystyrene-divinylbenzene Phase/Polymer Particle Platform |
| Length (Metric) | 15 cm |
| Technique | SEC |
| Max. Pressure | 55 bar |
| Product Line | TSKgel |
TSKgel™ Amide-80 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™
Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products.
| Pore Size | 100 Å |
|---|---|
| Particle Size | 3 μm |
| Stationary Phase | Carbamoyl |
| Temperature | 10°C to 50°C |
| Max. Pressure | 200 bar |
| Product Line | TSKgel |
| USP Type | L68 |
Thermo Scientific™ Accucore™ RP-MS HPLC Columns
Obtain an excellent combination of speed and quality of separation using these solid core columns that are optimized for LC-MS and UHPLC-MS methods.
| Pore Size | 80 Å |
|---|---|
| Particle Size | 2.6 μm |
| Carbon Load | 7% |
| Phase | Reversed Phase |
| Endcapped | Yes |
| pH | 2 to 9 |
| Surface Area | 130 m²/g |
| Particle Shape | Solid Core |
MilliporeSigma™ LiChroCART™ Columns/Purospher™ STAR Si Sorbents HPLC Cartridges, 5μm
Offers exceptional separation efficiency for normal-phase chromatography of low molecular weight compounds soluble in organic solvents
| Pore Size | 120 Å |
|---|---|
| Particle Size | 5 μm |
| Type | Cartridge |
| Stationary Phase | Silica |
| Phase | Normal Phase |
| Packing Material | Silica |
| Column Type | Normal Phase |
| pH | 2 to 7.5 |
| Quantity | 1/Pk. |
| Surface Area | 330 m²/g |
| Max. Pressure | 2175 psi (150 bar) to 2900 psi (200 bar) |
| USP Type | L3 |
| Column Format | Cartridge |
MilliporeSigma™ Supelco™ Ascentis™ Si HPLC Column, 5 μm Particle Size
The Ascentis™ Si is a high loading capacity silica with excellent peak shape. The Ascentis™ Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.
| Pore Size | 100 Å |
|---|---|
| Particle Size | 5 μm |
| Phase | Reversed Phase |
| Matrix | Silica Gel High Purity, Spherical/Silica Phase |
| Endcapped | No |
| pH | 2 to 6 |
| Technique | Fused-Core HPLC |
| Surface Area | 450 m2/g |
| Product Line | Ascentis |
TSKgel™ SP-NPR HPLC Column, 2.5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel SP-NPR columns are packed with non-porous, hydrophilic polymer beads which are surface modified with a strong cation exchanger. They are used for the separation and analysis of proteins and peptides.
Thermo Scientific™ ProPac™ Elite WCX HPLC Column
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This column provides consistent separations of therapeutic proteins including monoclonal antibodies, antibody drug conjugates, biosimilars and charge variants.
| Pore Size | Nonporous |
|---|---|
| Stationary Phase | Carboxylate |
| Temperature | 10°C to 60°C |
| Phase | Ion Exchange |
| Column Type | Ion Exchange |
| Green Features | FSC |
| Max. Pressure | 4500 psi (310 bar) |
| Column Format | Analytical Column |
| Particle Size | 5 μm |
| Packing Material | Nonporous DVB Particles with Hydrophilic Coating |
| Mobile Phase Compatibility | Recommended: Goods Buffers and NaCl. Minimum 20 mM salt required. Do not rinse with pure DI H2O |
| pH | 2 to 12 |
| Technique | Bio-LC |
| Product Line | ProPac |